SpinOculation是一種運用離心力輔助病毒與細胞結合藉以提高基因轉殖(Gene Transfer)效率的技巧,主要使用在懸浮性細胞如T細胞、B細胞的慢病毒轉導(Lentiviral Transduction)1

Sepax C-Pro現針對SpinOculation開發出自動化方案,能夠自動進行細胞濃縮、洗滌、添加病毒、離心以及細胞萃取行程,單一程序中即可完成大批量的病毒轉導。全程自動化、封閉式、規格化,能夠減少人力操作時間、整體處理時間、所需的病毒載體量並降低可能的微生物污染風險2

參考資料:
1. Lentivirus Transduction Protocol: Infecting your target cells, Applied Biological Materials
2. Remley, V.A., Jin, J., Sarkar, S. et al. High efficiency closed-system gene transfer using automated spinoculation. J Transl Med 19, 474 (2021). https://doi.org/10.1186/s12967-021-03126-4
3. SpinOculation C-Pro Protocol Software – SEPAX C-PRO CELL PROCESSING SYSTEM
4. 慢病毒載體(Lentiviral Vectors)指引 – 衛生福利部疾病管制署
5. Closed and automated CAR T cell lentiviral transduction by spinoculation, Cytiva